Application
Useful for kinase assay and Western blot.
Biochem/physiol Actions
Recombinant full-length human ERK3 (MAPK6) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. ERK3 (MAPK6) or mitogen-activated protein kinase 6 is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases) which also known as extracellular signal-regulated kinases (ERKs) that are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals and tyrosine phosphorylated in response to insulin and NGF (1). ERK3 is highly expressed in various human tissues, most abundantly in skeletal muscle (2).
Legal Information
PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC
Reconstitution
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Storage and Stability
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
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